Collagen-Based Bioactive Bromelain Hydrolysate from Salt-Cured Cod Skin

Considerable amounts of fish processing by-products are discarded each year. About 30% this material may be skin and bone. Fish has more than 80% its total protein content as collagen. Furthermore, in recent years, there been a growing demand for collagen-based peptides due to their beneficial health effects. So, the objective present study was optimise obtaining bioactive hydrolysates from salt-cured cod using protease Bromelain at 0.5% (w/w) concentration. This developed sustainable process that consumes less time energy uses an alternative source raw material. In addition, bromelain allows with important antioxidant (ORAC, 514 ?mol Trolox Equivalent/g protein) antihypertensive activities (inhibition ACE, IC50 166 ?g protein/mL) well excellent biocompatibility dermal subcutaneous cells.